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    The first step catalyzed by GTP cyclohydrolase I (GCHI ; FolE) is shared with the tetrahydrofolate synthesis pathway ( 10 and then three enzymes6-carboxy-5,6,7,8-tetrahydropterin synthase (QueD 7-carboxy-7-deazaguanine synthase (QueE and 7-cyano-7-deazaguanine synthase (QueC)lead to the formation of the preQ0 moiety ( 11, 12 ) ( Fig. 1 A ). Fig. 1. Biosynthesis of 7-deazaguanine derivatives. ( A ) The biosynthetic pathways to queuosine and archaeosine in tRNA. ADG, 7-amido-7-deazaguanine; aTGT, archaeal TGT; CDG, 5-carboxydeazaguanine; CPH4, 6-carboxytetrahydropterin; G, archaeosine; GCHI, GTP cyclohydrolase I (FolE H2NTP, Dihydroneopterin phosphate; TGT, tRNA-Guanine transglycosylase. (.

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